Cloning and expression and immunogenicity of Helicobacter pylori BabA2 gene

Abstract

Aim: To construct a recombinant strain which expresses BabA of Helicobacter pylori (H pylori) and to study the immunogenicity of BabA. Methods: BabA2 DNA was amplified by PCR and inserted into the prokaryotie expression vector pET-22b (+) and expressed in the BL21 (DE3) E. coli strain. Furthermore, BabA immunogenicity was studied by animal test. Results: DNA sequence analysis showed the sequence of BabA2 DNA was the same as the one published by GenBank. The BabA recombinant protein accounted for 34.8% of the total bacterial protein. The serum from H pylori infected patients and Balb/c miced immunized with BabA itself could recognize rBabA. Conclusion: BabA recombinant protein may be an potential vaccine for control and treatment of H pylori infection. Copyright © 2004 by The WJG Press.