Synthesis and spectral characterization of the phenothiazine-thiosemicarbazide probe for the optical solid-state detection of Hg2+ and Cu2+

Abstract

In this study, a phenothiazine-thiosemicarbazide (PTZDS) probe was synthesized and characterized. The synthesized PTZDS probe exhibited a yellow color, with a native fluorescence emission at λemission = 550 nm and λexcitation = 450 nm. Over other metal ions, the probe exhibited significant selectivity and sensitivity towards Hg2+ and Cu2+. The probe showed fluorescence quenching along with a minor shift in the absorbance spectra from 400 to 450 nm and 430 nm in the presence of Hg2+ and Cu2+, respectively. In addition, the color of the synthesized probe remarkedly faded with the addition of Hg2+ or Cu2+. Fluorescence measurements, infrared spectroscopy (IR), and density functional theory studies were employed to elucidate the binding process in the PTZDS + Cu2+ and PTZDS + Hg2+ sensor systems. Furthermore, photophysical investigations of the synthesized probe with Hg2+ and Cu2+ were performed. Finally, the probe was successfully employed as a solid-state thin layer chromatography (TLC) optical sensor for detecting Hg2+ and Cu2+ ions.