P350 Oropharyngeal candidiasis in HIV infected patients: associated risk factors and antifungal susceptibility testing of candida species by disc diffusion method

Abstract

Poster session 3, September 23, 2022, 12:30 PM-1:30 PM Objectives:-To isolate and identify Candida species by the standard microbiological method.-To estimate the frequency of oral Candidiasis according to age, sex, and other factors [CD4 + count, ART, tobacco consumption, diabetes, upper respiratory tract infection (URTI) , recent antibiotic consumption] in HIV-positive patients.-To determine the Antifungal Susceptibility pattern of the Candida species by Disc Diffusion Method. Methodology: A hospital-based cross-sectional study was conducted for 6 months in the Department of Microbiology of Sukraraj Tropical and Infectious Disease Hospital, Kathmandu, Teku, Nepal. A total of 408 HIV-positive patients attending the ART center of the hospital of different age and sex were included in the study. Study variables like CD4 + count, tobacco consumption, diabetes, URTI, and recent antibiotic consumption were noted. Standard microbiological guidelines were followed for the oral swab collection and transportation. Specimen was seeded on SDA agar plates (SDA with chloramphenicol and cycloheximide). Preliminary identification of the Candida was done on the basis of colony characteristics, gram staining, and germ tube test. Further for the speciation of Candida species, HiCrome TM Candida Differential Agar (HImedia, India) was applied where on the basis of coloration and colony morphology speciation was done. Similarly, Cornmeal Agar (Dalmau plate culture technique) and sugar Fermentation test were applied to confirm the Candida species. Muller-Hinton agar supplemented with 2% glucose and 0.5 μg/ml methylene blue dye (GMB) medium was used to perform the antifungal susceptibility testing of Candida isolates. Antifungal susceptibility testing (AST) of isolates was performed and interpreted according to Clinical and Laboratory Standards Institute (CLSI) M44-A document. Candida albicans ATCC 90028 was taken as the reference strain. All the data were entered in Microsoft excel and Chi-square was used to find out the association between the frequency of oral candidiasis and study variables. Results: Among 408 studied subjects, 225 (55.1%) were males and 183 (44.9%) were females. Of the participants, 116 (28.