The double staining of plant peroxidase and other proteins in the same polyacrylamide gel

Abstract

We present a simple and rapid technique for the double staining of plant peroxidase and other proteins in the same polyacrylamide gel using the principle of iodide oxidation followed by Coomassie Blue counterstain. The colored bands of peroxidase isozymes and proteins are easily distinguishable. An additional benefit of the method is the use of the low cost chemicals, as well as it eliminates the need for a potentially hazardous reagents frequently used in the detection of peroxidase isozymes.