Simple synthesis of biogenic Pd–Ag bimetallic nanostructures for an ultra-sensitive electrochemical sensor for sensitive determination of uric acid

Abstract

Bimetallic nanomaterials have potential catalytic behaviour in hydrogenation, clean- energy production, catalysis and sensors due to their great stability, loftier activity unique electrical and chemical properties. Herein, we prepared Pd–Ag bimetallic nanoparticles synthesized by using fungal extracted aqueous method, which is environmentally friendly cost-effective and simple procedure. The fabricated Pd–Ag bimetallic nanoparticles were investigated by small area electron diffraction (SAED), transmission electron microscopy (TEM) X-ray photoelectron spectroscopy (XPS) and energy dispersive X-ray spectroscopy (EDX) analysis. The electrochemical response of uric acid (UA) at Pd-Ag/CPE was studied in 0.1 M phosphate buffer solution at various pH, concentration and scan rate was investigated. Compare to Bare CPE, the Pd–Ag nanocomposite modified electrode displayed the highest electrocatalytic activity for the detection of UA A linear response in the range of 4.69–273 nM with remarkable detection limit of 5.543 nM (CDL = 3ϭ/M) and quantification limit of 16.64 nM (CQL = 10ϭ/M) was obtained. The established nanoparticles (Pd–Ag) embedded with carbon paste electrode (Pd-Ag/CPE) makes a good analytical tool for the sensing of UA in the biological and pharmaceutical samples.