Prevalence of Listeria monocytogenes, E. coli, Salmonella Spp. and Staphylococcus aureus Bacteria Contamination on Meat at Public Market in the North of Vietnam

Abstract

480 (four hundred and eighty) samples of beef, pork, and poultry sold at public markets in Bac Giang, Tuyen Quang, Lang Son and Thai Nguyen were collected in the two years of 2015 and 2016 to determine the contamination of Listeria monocytogenes, Staphylococcus aureus, E. coli, and Salmonella spp. the results showed that: The beef samples contaminated with Listeria monocytogenes was 10.0%; Staphylococcus aureus 21.6%, E. coli 65.0%; and Salmonella spp. 15.0%; The pork samples contaminated with Listeria monocytogenes was 11.6%, Staphylococcus aureus 20.0%, E. coli 63.3%; and Salmonella spp. 11.6%; The poultry samples contaminated with Listeria monocytogenes was 8.3%; Staphylococcus aureus 15.0%, E. coli 43.3%, and Salmonella spp. 10.0%. Staphylococcus aureus bacteria bearing genome sequence of enterotoxin typical B accounted for 79.4%; verotoxin-producing of Escherichia coli genome sequence of VT1 (verotoxin 1) accounted for 11.8%, and VT2 (verotoxin 2) accounted for 9.4%; Stn (heat-stable enterotoxin gene) enterotoxin genome sequence of Salmonella spp. accounted for 78.2%, InvA (Invasion gene A) 60.8%; hlyA (listeriolysin O-encoding gene) genome sequence of Listeria monocytogenes contamination on beef accounted for 21.8% (beef), 22.5% (pork), 34.3% (poultry). The Listeria monocytogenes were resistant to amoxicilline (77.7%), nitrofurantoin, ceftazidime, and oxytetracycline (3.7%), and 11.1% were resistant to Erythromycin; Staphylococcus aureus were resistant to amoxicilline (82.7%), nitrofurantoin (4.3%), erythromycin (15.5%), and 3.4% were resistant to ceftazidime; E. coli were resistant to ceftazidime (3.7%), kanamycin (7.5%), rifampicin (6.3%), bacitracin (48.1%), and 1.2% were resistant to oxytetracycline; Salmonella spp. bacteria isolates were resistant to kanamycin, ceftazidime (6.5%), and 63.0% were resistant to bacitracin.