Sesquiterpenoids from Stem Bark of Chisocheton lasiocarpus and Their Cytotoxic Activity against MCF-7 Breast Cancer Cell

Abstract

Sesquiterpenoids are derivatives of terpenoids that have a diverse skeleton and broad spectrum of biological activities, particularly for anticancer activity. The existence of sesquiterpenoids can be found in higher plants, especially in Chisocheton genera of the Meliaceae family. This research reported the isolation and elucidation structure of sesquiterpenoid from Chisocheton lasiocarpus stem bark, as well as their cytotoxic activity. The n-hexane extract was separated and purified by various chromatography techniques to obtained four sesquiterpenoids 1-4. The chemical structure of all compounds was identified by spectroscopic analysis, including IR, MS,1H-NMR,13C-NMR and DEPT, and compared with previous reported spectral data. These sesquiterpenoids were identified as eudesm-4(15)-ene-1β,6α-diol (1), allo-aromadendrane-10α,14-diol (2), allo-aromadendrane-10β,14-diol (3) and guaianediol (4), which led to the first report of sesquiterpenoid from Chisocheton lasiocarpus. The cytotoxic activity of the isolated compounds against MCF-7 breast cancer line were examined by using the resazurin method. The results showed eudesm-4(15)-ene-1β,6α-diol (1) shown the highest cytotoxic activity with IC50 108.08 ± 0.58 µM.