Molecular detection of Colletotrichum spp. on postharvest commodities of horticulture in Central Java and Yogyakarta, Indonesia

Abstract

Postharvest commodities are easily damaged by pathogen infection whether in the field or during the handling process. One of the important pathogens of a postharvest commodity is Colletotrichum spp. complex. Morphologically among species Colletotrichum spp. are difficult to be distinguished. This study aimed to detect Colletotrichum spp. from some postharvest commodities molecularly using specific primers C. gloeosporioides (CgInt, ITS4), C. acutatum (CaInt2, ITS2) and C. capsici (CcapF, CcapR). Isolates were taken from Yogyakarta Province and Temanggung Regency, covering 11 commodities infected by Colletotrichum spp. The result of molecular detection indicated that the six isolates were Colletotrichum gloeosporioides and ten isolates are Colletotrichum acutatum, while the remaining isolates are unknown.