Determination of nitrogen in proteins by means of indanetrione hydrate

Abstract

THE determination of nitrogen in organic substances has been performed by either of the well-established methods, namely, one attributed to Dumas or the other first described by Kjeldahl. These methods require approximately 300 μgm. of nitrogen for accurate determination. The method to be described requires much less material, and compares favourably in accuracy with these standard methods. The sample is decomposed by digestion in sulphuric acid, and the ammonia is determined quantitatively by means of indanetrione hydrate. © 1959 Nature Publishing Group.