OPTICAL RESOLUTION OF 2,6-DIAMINOPIMELIC Acid stereoisomers by high performance liquid chromatography for the chemotaxonomy of actinomycete strains

Abstract

For the chemotaxonomy of actinomycetes, we describe the optical resolution method for 2,6-diaminopimelic acid (DAP) stereoisomers by high performance liquid chromatography (HPLC) using 2,3,4,6-tetra-O-acetyl-B-D-glucopyranosyl isothiocyanate (GITC). The analysis was applicable both to whole-cell preparations and to cell wall ones. This method made it possible to analyze quantitatively ll-, dd-, and meso-DAP, compared with the previously reported methods of paper chromatography, thin-layer chromatography and HPLC. © 1989, Applied Microbiology, Molecular and Cellular Biosciences Research Foundation. All rights reserved.