Chiral recognition ability of cellulose derivatives bearing pyridyl and bipyridyl residues as chiral stationary phases for high-performance liquid chromatography

Abstract

Cellulose derivatives bearing pyridyl and bipyridyl residues were synthesized, and their recognition abilities as chiral stationary phases for high-performance liquid chromatography were evaluated. Compared with cellulose derivatives bearing these residues at the 2-, 3- and 6-positions of a glucose ring, the regioselectively substituted derivatives exhibited relatively high chiral recognition. The recognition ability of the derivatives was significantly influenced by the coordination of a Cu(II) ion to the bipyridyl residues. In addition, the derivatives were also used for ligand-exchange chromatography with an eluent containing a copper salt to directly separate amino acids without derivatization. © The Society of Polymer Science, Japan (SPSJ). All rights reserved.