Haematological parameters of Catfish (Clarias sp.) fed by immunostimulant added with Cr+3-Yeast (Saccaromices cerevisiae) and Garlic

Abstract

A study on the effect of immunostimulant feed with additional Cr3+-Yeast (Saccharomyces cerevisiae) and Garlic on Haematological Parameters of Catfish (Clarias sp.) has been conducted. There were two types of immunostimulant, a commercial feed combined with Cr3+-Yeast and a feed in combination of commercial feed, Cr3+-yeast, and garlic. A 180 Catfish with an average of 8.79 g in weight and 11 cm in length were used in the study. Haematological parameters measured were haematocrit level, total leucocyte, phagocytic index, and leucocyte differential. Haematological parameters were observed at day 0, 7, 14 after immunostimulant application, and also one and 5th days after challenge test. Catfish fed with immunostimulant Cr3+-Yeast and garlic combination show higher but not significantly different (P>0.05) haematocrit level (20.5%) than those fed with Cr3+-Yeast added immunostimulant (20%). A significantly higher (P<0.05) total leucocyte on immunostimulant added with Cr3+-Yeast and Garlic (58.850 sel/mm3) than Cr3+-Yeast (41.075 sel/mm3) and only feed (23.650 sel/mm3). Phagocytic index were observed significantly higher (P<0.05) on Cr3+-Yeast-Garlic (22%) than Cr3+-Yeast (13%). Leucocyte differential were observed lower in monocyte (4%), higher in neutrophil (23%) and highest in lymphocyte (73%) on Cr3+-Yeast-Garlic combination compared to Cr3+-Yeast combination (14% monocyte, 70.5% lymphocyte and 15.5% neutrophil).