PERFORMANCE AND MOLECULAR IDENTIFICATION OF BACTERIA ISOLATED FROM THE GUT OF BROILER BIRDS AFTER ANTIBIOTIC ADMINISTRATION AND ENZYME SUPPLEMENTATION

Abstract

This study evaluated the effect of feed additives (antibiotic or enzyme) on performance and bacteria population in the ileum of one day – old broiler chicks (ANAAC 2000) randomly distributed to three treatments having eight replicates and ten birds per replicate. Completely randomized design was used and experiment lasted for 35 days. Maize–soybean meal diet without antibiotic administration or enzyme supplementation served as control and was the same diet for all treatments. Antibiotic (Dicoxin plus ®) was administered to birds fed diet 2 and enzyme (Roxazyme G 2G ®) was supplemented to diet 3.Bacterial specific primers for rRNA gene sequences were used to amplify bacterial genes from samples and sequenced. Bacteria were identified from the gene sequences using Basic Local Alignment Search Tool (BLAST) against the National Centre for Biotechnology Information (NCBI). Enzyme supplementation significantly (P