Dynamic changes in scope for heart rate and cardiac autonomic control during warm acclimation in rainbow trout

Abstract

Time course studies are critical for understanding regulatory mechanisms and temporal constraints in ectothermic animals acclimating to warmer temperatures. Therefore, we investigated the dynamics of heart rate and its neuro-humoral control in rainbow trout (Onchorhynchus mykiss L.) acclimating to 16°C for 39 days after being acutely warmed from 9°C. Resting heart rate was 39 beats min-1 at 9°C, and increased significantly when fish were acutely warmed to 16°C (Q10=1.9), but then declined during acclimation (Q10=1.2 at day 39), mainly due to increased cholinergic inhibition while the intrinsic heart rate and adrenergic tone were little affected. Maximum heart rate also increased with warming, although a partial modest decrease occurred during the acclimation period. Consequently, heart rate scope exhibited a complex pattern with an initial increase with acute warming, followed by a steep decline and then a subsequent increase, which was primarily explained by cholinergic inhibition of resting heart rate.