Isozyme-specific interaction of protein kinase Cδ with mitochondria dissected using live cell fluorescence imaging

Abstract

Background: PKCδ signaling to mitochondria affects cellular apoptosis and metabolism. Results: A structure-function study using FRET-based imaging reveals that PKCδ binds to and is active at mitochondria via multiple isozyme-specific determinants. Conclusion: Determinants unique to PKCδ drive an interaction with mitochondria distinct from canonical PKC translocation to membranes. Significance: Isozyme-specific interaction of PKCδ with mitochondria constitutes a novel recruitment mechanism and increases mitochondrial respiration. © 2012 by The American Society for Biochemistry and Molecular Biology, Inc.