Comparing progesterone in blubber and serum to assess pregnancy in wild beluga whales (Delphinapterus leucas)

Abstract

Blood and blubber were sampled from live-captured beluga whales in Bristol Bay, Alaska. Progesterone levels were used toassess pregnancy status. For most cases, blubber progesterone levels correlated with serum, indicating that pregnancy can be assessed using a remote biopsy and thereby providing a valuable tool to better understand reproduction dynamics from populations that cannot be readily captured for examination. The Cook Inlet population of belugawhales (Delphinapterus leucas) is listedasendangeredandcontinues to decline for largely unknown reasons; however, there is some evidence that poor reproductive success is a contributing factor. Pregnancy is difficult to detect through observation, and, there is reluctance to capture endangered beluga whales for reproductive tract imaging via ultrasound or to obtain suitable samples for pregnancy assessments. An endocrine analysis of blubber biopsies collected by remote darting could represent a minimally invasive way to identify pregnant females and compare pregnancy rates among years or populations. Studies have validated the use of blubber biopsies to identify pregnant females in other cetacean species, but not beluga whales; therefore, validation of blubber progesterone levels to proven tests that reliably detect pregnancy was needed for this species. As part of a larger study, we sampled blood and blubber from live-captured beluga whales (21 females, 26males) in Bristol Bay, Alaska. Progesterone levels were determined in serum samples obtained from all animals and in blubber samples from a subset (14 females, 13 males) to determine pregnancy status, estimate the stage of pregnancy, and evaluate the suitability of using blubber alone for these assessments. In general, there was distinct separation of high levels of progesterone in serum and blubber for presumed pregnant females and low levels for males and presumed non-pregnant females. Blubber progesterone levels in two females (14% of females tested) were intermediate (i.e. ambiguous); their corresponding serum levelswere consistentwith being pregnant in one case and not being pregnant in the other. Except for these two intermediate values, pregnancy status of beluga whales could be determined fromblubber alone, thereby providing a valuable tool to better understand reproduction dynamics from populations that cannot be captured for examination.