Visual space from visual motion: Turn integration in tethered flying Drosophila

Abstract

Organisms navigating by path integration need to continuously measure their forward movement and their angular orientation with respect to an external reference. How they do it is little understood. Tethered flies at the flight simulator 'navigate' in an artificial visual landscape without forward movement. They can return to a previously held orientation if the panorama provides a singularity (landmark) as reference. Surprisingly, in a regularly striped drum without singularities, they can use a temporal cue instead. In this experiment the arena is illuminated with only one color that is either green or blue. The arena is virtually divided into four quadrants. Whenever a quadrant boundary moves past an arbitrary point, the color of the arena light changes. When a fit is heated with one color it acquires a preference for the other one. Subsequently, it avoids the borders toward the potentially 'hot' quadrants even without touching them. The only way to achieve this is by turn integration, that is, by adding and subtracting all the turns it performs once it crosses the border. The color switch defining the border crossing resets the turn integrator, using the orientation of the arena at this moment as reference. In contrast, landmarks or, if it were available, the skylight compass enable the fly to establish by pattern learning any orientation as a reference. If the reference orientation coincides with the desired orientation, that is, if the animal stores the pattern while being oriented toward the goal, it can maintain its orientation without recourse to turn integration (which may be error prone).