Correlation between Serum Anti-Apoptotic Bcl-2 Level and its Immunohistochemical Expression in Relation to Apoptosis in Gastric Cancer

  • H. Fatani S
  • H. Mukhtar M
  • S. Ali A
N/ACitations
Citations of this article
9Readers
Mendeley users who have this article in their library.

Abstract

Introduction: Gastric cancer (GC) remains a major public health problem worldwide being the third commonest cause of cancer death worldwide. Many recent studies focus on the immunohistochemical evaluation of Bcl-2 expression and its serum expression and its prognostic significance in gastric cancer each one separately. We conducted our study to determine the correlation between serum Bcl-2 antigen and gastric carcinoma, to investigate whether serum Bcl-2 concentrations can be used as marker for immunohistochemical determination of Bcl-2 alterations in gastric cancer patients and examine the association between its expression and other clinicopathological parameters. Methods: Our study conducted in Forty-five consecutive patients with gastric cancer underwent gastrectomy in Mansoura Gastroenterology Center. ELISA was used for the estimation of serum Bcl-2 levels in patients with different stages of gastric cancer. Immunohistochemical localization of Bcl-2 antigen was performed on formalinfixed, paraffin-embedded tissue block; Bcl-2 expression was detected from mild to moderate apoptotic index (AI). Results: Positive serum Bcl-2 expression was found in 13/45 patients (28.9%). Bcl-2 protein was immunohistochemically localized in the cytoplasm of 45% (18/40) of gastric cancer patients. Total apoptosis positivity in Bcl2 detected immunohistochemicaly was detected in 45% (18/ 40) of cases; while total apoptosis positivity in serum expression of Bcl-2 was detected in 28.9% (13/ 45). Conclusion: our current study demonstrated that the formation and growth of cancer is a complex process that requires further research in correlation with the results assessed between serum and immunohistochemical expression of Bcl-2 and with its role in the process of apoptosis.

Cite

CITATION STYLE

APA

H. Fatani, S., H. Mukhtar, M., & S. Ali, A. (2015). Correlation between Serum Anti-Apoptotic Bcl-2 Level and its Immunohistochemical Expression in Relation to Apoptosis in Gastric Cancer. Journal of Molecular Biomarkers & Diagnosis, 06(06). https://doi.org/10.4172/2155-9929.1000248

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free