Chemical composition and antioxidant activity of essential oil, various organic extracts of Cistus ladanifer and Cistus libanotis growing in Eastern Morocco

Abstract

In the present work, we studied the chemical composition of the essential oil of Cistus ladanifer and Cistus libanotis growing in Eastern Morocco. The essential oils were obtained by hydrodistillation and their chemical composition was analysed using gas chromatography-mass spectrometry (GC-MS). Camphene, borneol, cyclohexanol-2, 2, 6 tremethyl, terpineol-4 and alpha -pinene were the main constituents of the essential oil of C. ladanifer, while in the essential oil of C. libanotis we obtained terpineol-4, gamma -terpinene, camphene, sabinene, alpha -terpinene and alpha -pinene. The antioxidant potential of various extracts (water, ethanol, ethanol:water (50:50), methanol, methanol:water (50:50), acetonitrile) and essential oils of C. ladanifer and C. libanotis were carried out by the method of 1,1-diphenyl-1-picrylhydrazyl-hydrate (DPPH) free radical scavenging. Total phenolic and flavonoid contents were determined. The result show that C. ladanifer of the leaves of methanol:water (50:50) extract had the highest value of total phenolic content and the lowest was present in ethanol:water (50:50) extract of the stem and acetonitrile extract of the flowers of C. libanotis. From our experimental results, the extract of flowers, fruit, stem and leaves of those plants showed highest potential as free radical scavengers.