Fabrication and Characteristic of ZnO Glucose Oxidase Enzyme Electrode Based on Flexible Substrate

Abstract

Well-aligned ZnO nanorods (NRs) were grown on the ZnO seed layer of a polyimide (PI) coated flexible substrate using the hydrothermal method and used as a support matrix for the immobilization of glucose oxidase (GOx). The crystal structures and surface morphologies of the so-formed ZnO nanorods were analyzed by X-ray diffraction (XRD) and scanning electron microscopy (SEM). GOx was immobilized on the surfaces of ZnO nanorods using electrostatic adsorption. Both the GOx and modified ZnO nanorods were characterized by UV-visible spectroscopy, and the absorption peaks of ZnO and GOx can be detected. Fourier transform infrared (FTIR) spectroscopy were used to characterize the chemical structure of the glucose oxide, which still maintained its biological activity. This study provides an experiment basis for the preparation of flexible glucose biosensors owing to the flexibility of the enzyme electrode, which showed a sensitive current response when tested by cyclic voltammetry. © Editorial office of Acta Physico-Chimica Sinica.