Phytochemical evaluation and pharmacological screening of ethanolic and aqueous leaf extracts of Pupalia lappacea for antihyperlipidemic and antihyperglycemic activities in alloxan induced diabetic Albino wistar rats

Abstract

Background: Diabetes is associated with damage to the liver, pancreas and kidney. The damage may vary among patients. This study assessed the hypoglycaemic and antihyperlipidemic activities of leaves ethanolic and aqueous extracts of Pupalia lappaceae in alloxan induced diabetic albino wistar rats.Methods: There were Fifty four rats divided into nine groups containing six rats each. Group 1 consists of normal rats that were given only normal saline and served as a control group. Group 2 consists of normal rats that were given alloxan monohydrate (150mg/kg b.w). Group 3 consists alloxan induced diabetic rats that were given daily sterile solution. glibenclamide+simvastatin (5mg/kg) Group 4 consists of alloxan induced diabetic rats that were given daily sterile solution, AEPL extract (100mg/kg). Group 5 consists of alloxan induced diabetic rats that were given daily sterile solution, drug extract AEPL (200mg/kg). Group 6 consists of alloxan induced diabetic rats that were given daily sterile solution, drug extract AEPL (400mg/kg) Group 7 consists of alloxan induced diabetic rats that were given daily sterile solution, drug extract EEPL (100mg/kg), Group 8 consists of alloxan induced diabetic rats that were given daily sterile solution, drug extract EEPL (200mg/kg) Group 9 consists of alloxan induced diabetic rats that were given daily sterile solution, drug extract EEPL (400mg/kg respectively for 21days by an intragastric tube with free access of food and water.Results: Chemical composition of the plant was estimated by GCMS technique several biochemical parameters were assessed. Oral administration of the extract resulted in significant reduction in mean values of blood glucose, triglycerides, total cholesterol, cholesterol ratio, LDL, VLDL, accompanied by increase in the mean value of the HDL in diabetic rats and histopathology of liver, pancreas, and kidney showed significant changes.Conclusions: The effects produced were closely similar to standard antidiabetic and antihyperlipidemic drug. It can thus be concluded that the ethanolic and aqueous extract of Pupalia lappaceae exhibit antihyperlipidemic and antihyperglycemic activities in alloxan induced diabetes in rats.