Transesterification synthesis of high-yield biodiesel from black soldier fly larvae using the combination of Lipase Eversa Transform 2.0 and Lipase SMG1

Abstract

The larvae of black soldier fly (BSFL) are rich in lipids, and in the current study, BSFL was used to produce biodiesel by enzymatitransesterification with methanol. Biodiesel obtained by free lipase-catalyzed transesterification is always with side-reactionTo address the limitation of free lipase, for the first time, Lipase SMG1 and Lipase Eversa Transform 2.0 were combined to usas catalysts at the same time. The effects of different reaction conditions on the yield of biodiesel were investigated, includinthe type of enzyme, reaction temperature, and molar ratio of BSFL to methanol. The molar ratio of BSFL to methanol 1:3one-step addition of methanol at 25 °C were selected as optimal conditions. The fatty acid methyl ester content achieved t98.45% after 8h reaction under the optimal conditions. Moreover, the main properties of the final biodiesel indicators met thEN14214 biodiesel standard.