Evaluation of Anti-Diabetic Activity of Silver Nanoparticles Synthesized from Ethanolic Extract of Phyllanthus niruri on Wistar Rats

Abstract

Diabetes is a metabolic disorder characterized by hyperglycemia resulting from either the deficiency in insulin secretion or the action of insulin Diabetes can affect numerous different organ systems in the body and, over time, can lead to serious complications. Complications are microvascular or macrovascular. To evaluate the anti-diabetic effect of Phyllanthus niruri silver nano-particles. Streptozotocin (STZ) of 50mg/kg is used to induce diabetes in wistar rats. The wistar rats were distributed to six different groups. Group-I represented as Control; Group-II represented as diabetic control (STZ ); Group-III represented as 5 mg/kg body weight of glibenclamide + diabetes; Group-IV represented as  phyllanthus niruri extract + diabetes; Group-V represented as 100 µg Phyllanthus niruri silver nanoparticles + diabetes; group-6- Phyllanthus niruri silver nanoparticles150µg + diabetes. The silver nanoparticles were inspected for the morphological and optical properties investigated using UV spectrophotometer, SEM and FT-IR techniques.  The AgNPs has shown characteristic peak at 428nm.The study revealed that the AgNPs treatment reduced serum blood glucose concentrations remarkable improvement in the body weight, Abnormal high serum levels in diabetes treatment with AgNp’s they reached to normal in STZ-challenged diabetic rats. The histo-pathological alterations were also studied in all the experimental animals.  The pathology results revealed that the AgNPs treatment induces the regeneration of islets cells of pancreas in the experimental rats. The research study proved that the Phyllanthus niruri AgNPs exerts anti-diabetic properties. Keywords: Diabetes, STZ, Phyllanthus niruri, silver nanoparticles