Genes encoding callose synthase and phytochrome A are adjacent to a MAP3Kα-like gene in beta vulgaris US H20

Abstract

MAP3K, a gene that encodes a key conserved protein kinase, is responsible for initiating a rapid cascade of cellular events leading to localized cell death. Hypersensitive response, as it is termed, enables genetically resistant plants to limit microbial invasion under the right environmental conditions. Since knowledge of close physically linked genes is important for genome analysis and possibly for improving disease resistance, systematic DNA sequence analysis, gene annotation, and protein BLASTs were performed to identify and characterize genes in close physical proximity to a MAP3K-like gene in Beta vulgaris L. US H20. On the same 125Kb BAC, callose synthase (BvCS) and phytochrome A (PhyA) genes were within 50Kb of MAP3K. The close physical linkage of these genes may result from selection for coordinated responses to disease pressure. Bert, a new chromodomain-carrying gypsy-like LTR retrotransposon, resides within an intron of the BvCS gene, where it is transcribed from the opposing strand. © 2011 L. David Kuykendall and Jonathan Y. Shao.