Identification and characterization of a novel inhibitor of alginate overproduction in Pseudomonas aeruginosa

Abstract

In this study, we performed whole-genome complementation using a PAO1-derived cosmid library, coupled with in vitro transposon mutagenesis, to identify gene locus PA1494 as a novel inhibitor of alginate overproduction in P. aeruginosa strains possessing a wild-type mucA. We report identification and characterization of a novel inhibitor of a mucoid biofilm produced by laboratory and clinical strains of Pseudomonas aeruginosa with a wild type MucA. © 2013 Federation of European Microbiological Societies.