Analisa Kandungan Flavonoid dan Aktivitas Antioksidan dari Rempah Tumbuhan Obat Sumatera Barat

Abstract

Antioxidant activity and total flavonoid content of five medicinal plant specieses of West Sumatera have been measured. The spesieses are Jahe (Zingiber officinale Rosc), Kunyit (Curcuma domestica Val), Kencur (Kaemferia galanga Linn), Lengkuas (Alpinia Galanga Linn), and Pala (Myristica fragrans Houtt). The total flavonoid content and antioxidant activity were measured on total ethanolic extract, liphofilic fraction and hydrophilic fraction of each species. The total flavonoid content was measured using colorimetric with alumunium chloride used as complexing agent and quercetin used as standard compound.The examination of antioxidant activity was carried out by spectrophotometry UV-Visible using DPPH reagent. The total flavonoid content of Jahe (Zingiber officinale Rosc), Pala (Myristica fragrans Houut), Kunyit (Curcuma domestica Val) were 0.85; 0.54; 19.77 µg/g respectively (quercetin equivalent).The result showed that these three plants have highest antioxidant activity. IC50 of jahe against 20 µg/ml DPPH were 80.62, 69.35, 109.98 µg/ml for total ethanolic extract, liphofilic fraction and hydrophilic fraction respectively. IC50 of ethanolic extract and hydrophilic fraction of kunyit were 68.21 and 47.09 µg/ml, while IC50 of ethanolic extract and hydrophilic fraction of pala were 50.08 and 71.67 µg/ml