Isolation and spectroscopic analysis of compounds from baccaurea racemosa (Reinw. ex blume) müll. arg. pulp

Abstract

The pulp of Baccaurea racemosa (Reinw. ex Blume) Müll. Arg. is reported to have active compounds with medicinal importance, such as anti-inflammatory and antioxidant activities. However, there has been no report of any isolated compounds from B. racemosa pulp. The present study aims to isolate and characterize the compounds from B. racemosa pulp and their antioxidant activity. The powdered pulp of B. racemosa was extracted using methanol and was partitioned using n-hexane, dichloromethane and ethyl acetate to get corresponding fractions. The ethyl acetate fraction was isolated using conventional column chromatography to obtain compounds 1 and 2. Based on the spectrometric analyses (1D-NMR, 2D-NMR, infrared and GC-MS), compounds 1 and 2 were identified as 5-Hydroxymethylenefurfural (5-HMF) and tartaric acid, respectively. The compound 5-HMF showed antioxidant activity with IC50 > 7.94 mM. This study is the first report related to the isolation and identification of compounds from B. racemosa pulp, therefore 5-HMF from B. racemosa may be potentially used as a candidate for natural antioxidants.