Rapid detection of brucella species in cattle semen by pcr

Abstract

To overcome the problems of sensitivity and specificity associated with serological tests and difficulty in isolation of bacteria for diagnosing brucellosis, a PCR assay was standardized using specific primer from the gene encoding 31 kDa protein for Brucella species with genomic DNA isolated from the overnight grown cultures of B. abortus S99. The PCR could detect the Brucella organism up to 40 cfu per ml in cattle semen. Reprecipitation of DNA with butanol increased sensitivity of PCR. © GSP, India.