Specific detection and localization of microsporidian parasites in invertebrate hosts by using in situ hybridization

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Abstract

We designed fluorescence in situ hybridization probes for two distinct microsporidian clades and demonstrated their application in detecting, respectively, Nosema/Vairimorpha and Dictyoceola species. We used them to study the vertical transmission of two microsporidia infecting the amphipod Gammarus duebeni. © 2013, American Society for Microbiology.

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Dubuffet, A., Smith, J. E., Solter, L., Alejandra Perotti, M., Braig, H. R., & Dunn, A. M. (2013). Specific detection and localization of microsporidian parasites in invertebrate hosts by using in situ hybridization. Applied and Environmental Microbiology, 79(1), 385–388. https://doi.org/10.1128/AEM.02699-12

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