Mapping of the mecillinam resistant, round morphological mutants of Escherichia coli

Abstract

Genes responsible for round morphology in mecillinam-resistant, round morphological mutants of E. coli have been mapped. Three mutants, called rodX, mapped at around 14 min, and two, called rodY, mapped at around 70 min by P1 transduction. These are either the same or very close to the loci reported, respectively, for the rodA and envB genes. This suggests that mecillinam can be used very efficiently to select for round morphological mutants of rodA and envB after nitrosoguanidine treatment.