ACUTE TOXICITY AND ANTIFUNGAL ACTIVITY OF THE OINTMENT Murraya koenigii ETHANOL EXTRACT

Abstract

Murraya koenigii (MK) plants are a type of plant whose bioactive compounds have been widely used in various countries as traditional medicines with metabolites containing alkaloids, flavonoids, tannins, steroids/triterpenoids and anthraquinones. The purpose of this study was to determine of acute toxicity effect and to test the antifungal effects of ointment MK ethanol extract. Acute toxicity testing is carried out with the initial stage of testing at a dose of <2000 mg/kgBW. The antifungal test was carried out by the diffusion disc method against Candida albicans by formulating the MK leaves extract into an ointment preparation. The results of testing the secondary metabolites of MK were alkaloids, flavonoids, tannins, steroid/triterpenoid, and anthraquinone. Toxicity testing was indicated by a decrease in body weight, but it was not seen in the MK extract because the weight difference did not show a difference of p>0.05. The formulated ointment was subjected to an organoleptic evaluation test, pH, spreadability, and homogeneity. MK leaves ethanol extract can be formulated into ointment because it is safe to use with an LD50 value >2000mg/kgBW. The concentrations of 12.5% and 25% were the best concentration to inhibit the growth of Candida albicans.