In-vitro ANTI-CERVICAL CANCER ACTIVITY OF ISOLATE ASE 3.3.3 FROM ETHYL ACETATE EXTRACT OF Annona squamosa L. LEAF

Abstract

The increasing burden of cervical cancer has urged medicinal scientists to innovate novel anticancer which can be derived from ethnomedicinal plants. Herein, ethyl acetate isolate of Annona squamosa L. leaf (ASE 3.3.3) was investigated for its anti-cervical cancer activity in vitro using HeLa cell lines. 2,2-Diphenyl-1-picrylhydrazyl (DPPH) scavenging assay was employed to measure the antioxidant potential of the extract. The results suggest that ASE 3.3.3 was active in scavenging the free radical (IC50=84.2 mg/L). The brine shrimp lethality test suggested its potential as an anticancer (LC50<30 mg/L). ASE 3.3.3 is potent in suppressing the proliferation of HeLa cell lines with IC50=1.003 mg/L. Squalene was detected in the isolate which could facilitate the anticancer activity. Taken altogether, ASE 3.3.3 is worth further exploration as an anti-cervical cancer agent.