Abstract
We describe the synthesis of a novel polyamino polycarboxylic ligand, its ability to coordinate metal-ions and attachment to a solid support designed for protein purification through Immobilised Metal-ion Affinity Chromatography (IMAC). The resin was found to be highly efficient for purification of His-tagged HCV E2 glycoproteins expressed in 293T mammalian cells. This journal is
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CITATION STYLE
Popescu, C. C., Stoian, M. C., Cucos, L. M., Coman, A. G., Radoi, A., Paun, A., … Matache, M. (2020). A polycarboxylic chelating ligand for efficient resin purification of His-tagged proteins expressed in mammalian systems. RSC Advances, 10(40), 23931–23935. https://doi.org/10.1039/d0ra02382e
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