Isolation and characterization of Lactobacillus brevis' surface layer protein (Slp) from Indonesian Culture Collection

Abstract

Lactobacillus brevis is a potential probiotic in functional foods, yet it is still not well explored. It has been studied to enhance calcium and other minerals absorption in intestine via surface layer protein (Slp). The protein forms mineral-Slp complex and then facilitates bacteria cells binding to the intestinal epithelial cells. Among four L.brevis isolates from Indonesian Culture Collection-Indonesian Institute of Sciences (InaCC LIPI) and one isolate from Food and Nutrition Culture Collection (FNCC Universitas Gadjah Mada), L.brevis B144 shows the highest concentration of Slp in aerated fermentation. The Slp from L.brevis B144 has 45% coverage of peptide mapping compared with that of in database (Lactobacillus brevis KB290). Furthermore, the isolated Slp which was analyzed with 2D SDS PAGE resulted four spots at acidic and neutral pI, namely approximately 3.8-3.9; 5.5-6.5; and 7.6-7.8.