Fast and convenient 5-hydroxymethylcytosine enrichment workflow for next-generation sequencing

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Abstract

The Scientific™ EpiJET™ 5-hmC Enrichment Kit is highly specific for different DNA samples containing 5-hydroxymethylcytosine (5-hmC), an extensively studied DNA epigenetic modification. This tool, when combined with next-generation sequencing, offers new ways of analyzing 5-hmC at the genomic level. The data show that this tool can be used for both locus-specific and whole genome– specific analysis, yielding 5-hmC distribution patterns across different genomes.

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Kaniušaitė, M., Astromskas, E., Alzbutas, G., Bružaitė, R., & Lagunavičius, A. (2015). Fast and convenient 5-hydroxymethylcytosine enrichment workflow for next-generation sequencing. Nature Methods, 12(2), I–III. https://doi.org/10.1038/NMETH.F.377

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