Rapid detection of FMO3 single nucleotide polymorphisms using a pyrosequencing method

Abstract

The present study aimed to develop a reliable pyrosequencing method to detect four single nucleotide polymorphisms (SnPs) of the flavin-containing monooxy- genase 3 (FMO3) gene and to compare the ethnic differences in their allelic frequencies. The pyrosequencing method was used to detect four FMO3 SnPs, namely, c.855c>T (n285n, rs909530), c.441c>T (S147S, rs1800822), c.923a>G (e308G, rs2266780) and c.472G>a (e158K, rs2266782). The allelic frequencies of these SnPs in 122 unrelated Korean subjects were as follows: i) 44.7% for c.855c>T; ii) 23.4% for c.441c>T; iii) 23.0% for c.923a>G; and iv) 27.1% for c.472G>a. linkage disequilibrium (ld) analysis revealed that the SnPs c.923a>G and c.472G>a exhibited a strong ld (d'=0.8289, r2=0.5332). in conclusion, the pyrosequencing method developed in this study was successfully applied to detect the c.855c>T, c.441c>T, c.923a>G and c.472G>a SnPs of FMO3.