The Saccharomyces cerevisiae AP-1 protein discriminates between oxidative stress elicited by the oxidants H 2O 2 and diamide

Abstract

The Saccharomyces cerevisiae AP-1 protein (yAP-1) is a key mediator of oxidative stress tolerance. Transcriptional activation by yAP-1 has been shown to be inducible by exposure of cells to H 2O 2 and diamide, among other oxidative stress eliciting compounds. Here we define the segments of the yAP- 1 protein that are required to respond to this environmental challenge. Western blotting analyses indicated that levels of yAP-1 do not change during oxidative stress. Deletion mutagenesis and gene fusion experiments indicate that two different segments of yAP-1 are required for oxidative stress inducibility. These two domains function differentially depending on the type of oxidant used to generate oxidative stress. Three repeated cysteine- serine-glutamate sequences located in the carboxyl terminus are required for normal regulation of yAP-1 function during oxidative stress. Replacement of these cysteine-serine-glutamate repeats by alanine residues does not similarly affect H 2O 2 and diamide regulation of yAP-1 function. While yAP- 1 transactivation is enhanced by exposure to either H 2O 2 or diamide, the protein responds to the oxidative stress produced by these compounds in nonidentical ways.