Physico-chemical and microbiological comparison of nystatin, amphotericin A And amphotericin B, and structure of amphotericin A

Abstract

Two polyene antibiotics, nystatin and amphotericin A, were compared by physico-chemical and microbiological methods. The two antibiotics were found to have thesame molecular weight, 926, by plasma desorption and electron-impact Ms. However, 13C Nmr spectrometry and Hplc studies indicated that the two molecules are different. The 200 Mhz Nmr studies indicated a chemical environment of 24 carbons of amphotericin A identical with that of the carbons of amphotericin and nystatin. © 1985, JAPAN ANTIBIOTICS RESEARCH ASSOCIATION. All rights reserved.