Development and characterization of 10 microsatellite markers from Wisteria floribunda (Fabaceae)

Abstract

Wisteria floribunda is a deciduous liana species widely distributed in Japan. It is essential to evaluate the clonal structure of this species because high clonal ability enables lianas to make dense colonies after canopy gaps occur. Therefore, we developed 10 microsatellite markers for W. floribunda from genomic shotgun sequences available in the publicly available database to study clonal structure and life history strategy of this species. The number of alleles per locus ranged from 7 to 16, with an average of 10.2. The observed and expected heterozygosity ranged from 0.483 to 0.926 and from 0.642 to 0.916, with averages of 0.769 and 0.800, respectively. These findings will contribute to the understanding of ecological roles and management of this species.