The effect of MnSO4 and unrefined sea salt on bioethanol production by lignocellulose degradation of oil palm empty fruit bunches (OPEFB) using Phlebia sp. MG-60

Abstract

This study aimed to determine the effects of MnSO4 and sea salt against the degradation lignocellulose and ethanol production generated from Oil Palm Empty Fruit Bunches (OPEFB) using Phlebia sp. MG-60. The factorial experiment in a randomized block design was used, consists of two factors: MnSO4 concentration (0, 300, and 600 mg/L), sea salt concentration (0%, 3%, and 5% (w/v). The results revealed that the addition of MnSO4 and sea salt to substrate, and its interaction were significantly different (ρ value < 0.05) on the change of total soluble phenols (mg/g), total reducing sugar (mg/g), pH, lignin concentration (%), weight loss (%) and ethanol concentration (%). The best treatment from this study was obtained from the addition of MnSO4 300mg/l, sea salt 3% incubated for 20 days. This treatment has the highest value of total reducing sugars (22.471 mg/g), total soluble phenols (0.334 mg/g), weight loss (16.23%), ethanol (0.71%), lignin (19.17%) and pH (6.1).