Impact of Asian bullfrog (Rana tigerina) skin oil on growth inhibition of colletotrichum gloeosporioides (in vitro) and its prediction modeling

Abstract

The effect of Asian Bullfrog (Rana tigerina) skin oil on the mycelium growth of pathogenic fungi, Colletotrichum gloeosporioides, was studied in vitro. A poisoned substrate technique method (dilution in solid media) was employed to evaluate the antifungal activity of the frog skin oil (FSO) at different concentrations (0-10 %) against C. gloeosporioides. FSO showed an inhibitory effect, and the efficacy increased with increasing FSO concentration. A modified logistic model was applied to evaluate the antifungal activity of FSO at different concentrations. The modified logistic model showed a good agreement with the observed growth data for C. gloeosporioides. It can be concluded that FSO could potentially control the growth of C. gloeosporioides and that the modified logistic model could describe the inhibitory pattern of FSO against C. gloeosporioides effectively.