Aspergillus niger Str 3 and Neurospora sitophila for phytase production on coconut oil cake supplemented with rice brand in solid-state fermentation

Abstract

The use of phytase as a feed supplement is getting popular. However, the production system of this enzyme should be optimized to reduce production cost. The objective of this study was to evaluate the ability of 10 fungi isolates i.e., Aspergillus niger (5 isolates), and Neurospora crassa InaCC F226 to produce phytase, and select best phytase producer for phytase production on coconut oil cake supplemented with rice brand in solid-state fermentation. A. niger Str3 and N. sitophila produced phytase of 4.6 and 3.4 unit respectively were selected for phytase production owing to its ability to produce phytase in submerging fermentation with glucose as the primary carbon sources. These potential isolates were then used for phytase production on coconut oil cake supplemented with rice brand in solid-state fermentation. The effect of inoculants type, initial moisture content, and additional carbon sources were evaluated to obtain the optimum condition for phytase production. Media contained coconut oil cake supplemented with rice brand at a ratio of 20 to 50% could be used for phytase production. Initial moisture content and incubation time affect phytase production. Optimum initial moisture content was about 60-70%. This work concludes A. niger and N. sithophyla were good inoculant for phytase production using formulated media contained coconut oil cake and rice brand in solid-state fermentation.