Menthol-carboxylic acid deep eutectic solvent for extraction of carotenoids from palm ester

Abstract

In this study, the carotenoids in palm biodiesel will be extracted using methanol and deep eutectic solvent (DES) as a co-solvent using the solvent extraction process. The DES used is menthol-based as a hydrogen bond donor and acetic acid and lauric acid hydrogen bond acceptor (HBA) and, which is correlated with a molar ratio of 1: 1 and 2: 1. To see the functional groups formed, FTIR characterized the DES generated while seeing the structure of the components formed in DES using H1NMR. To see the β-carotene portion obtained, complete carotenoids were characterized by the UV-Visible Spectrophotometer, HPLC. The optimum condition for complete carotenoid extraction using DES menthol is 366.3 ppm: lauric acid with an ester: DES ratio of 1: 0.5. Whereas the content of β-carotene derived from DES menthol is 14.7 ppm lauric acid. These findings show that the total extraction yield of carotenoids and β-carotene in palm methyl esters can be increased by DES as a co-solvent.