Comparison of osseointegration in areas grafted with deproteinized bovine bone and native bone. A preclinical study

Abstract

The aim of this study was to evaluate the osseointegration of implants placed in rat tibia sites grafted with Deproteinized Bovine Bone (DBB) and Native Bone (NB). Twenty-eight rats were divided into two groups according to the type of substrate in which the implants were to be placed: NB – implants placed in native bone; DBB – implants placed in areas grafted with DBB. In the DBB group, the bone defect was made and filled with the bone substitute 60 days before placing the implant. The animals were euthanized 15 or 45 days after implant placement. Osseointegration was assessed by the removal torque, volume of mineralized tissues around the implants (BV/TV), bone-implant contact (%BIC), and bone between threads (%BBT). The implants placed in NB presented higher removal torque (8.00 ± 1.26 Ncm vs. 2.33 ± 0.41 Ncm at 15 days and 22.00 ± 2.44 Ncm vs. 4.00 ± 1.41 Ncm at 45 days), higher %BV/TV (47.92 ± 1.54% vs. 33.33 ± 4.77% at 15 days and 70.06 ± 0.91% vs. 39.89 ± 5.90% at 45 days), higher %BIC (39.68 ± 5.02% vs. 9.12 ± 5.56% at 15 days and 83.23 ± 4.42% vs. 18.81 ± 7.21% at 45 days), and higher %BBT (34.33 ± 5.42% vs. 13.24 ± 8.72% at 15 days and 82.33 ± 3.13% vs. 22.26 ± 8.27% at 45 days) than the implants placed in DBB grafted areas. The degree of osseointegration was lower in implants placed in the area grafted with DBB than in NB in rat tibias.