The mucin box and signal/anchor sequence of rat neutral ceramidase recruit bacterial sphingomyelinase to the plasma membrane

Abstract

Sphingolipid metabolites act as lipid mediators in various cellular events. We found that the mucin box and signal/anchor sequence of a rat neutral ceramidase recruit bacterial sphingomyelinase to the plasma membranes of mammalian cells. The mucin box-fused sphingomyelinase hydrolyzed cellular sphingomyelin efficiently to generate ceramide.