Extraction procedure optimization of the method for detecting ethylene oxide and 2-chloroethanol in sesame seed

Abstract

This study describes modifications of the extraction procedure within the European Union Reference method for determination of ethylene oxide and 2-chloroethanol in sesame seed. The method suggests utilisation of uniform stainless steel balls in order to facilitate extraction in small seed samples. Experiment was conducted with combination of balls of different sizes and the extraction efficacy was assessed by measurement of 2-chloroethanol in sesame seed samples from the local market. Increased efficacy of 18.3% for ethylene oxide and 16.2% for 2-chloroethanolwas observed when the combination of two diameters of balls was used compared to samples extracted by the guidelines in original method and alternative approach with uniform balls of the lower diameter..