A Simple Modified DNA Extraction Method of Maize

Abstract

Marker Assisted Selection (MAS) is becoming an efficient method of crop improvement programme for maize and other crops. For this method, it requires quality genomic DNA extraction of the large sample. The present study described a simple, cost-effective miniprep DNA extraction method without using liquid nitrogen from the leaf of maize that can be performed in a laboratory with basic facilities. Using this method genomic DNA was extracted from lines derived from CAU-87. The ratio of absorbance at 260 nm to 280 nm ranges from 1.833 to 2.2 and the concentration of DNA (ng/µL) vary from 60ng/µL to105 ng/µL. The PCR amplification using three pairs of crtRB1 3'TEgene-specific primers yield an acceptable result.