Bradykinin stimulates NF-κB activation and interleukin 1β gene expression in cultured human fibroblasts

Abstract

Bradykinin (BK), a pluripotent nonameric peptide, is known for its proinflammatory functions in both tissue injury and allergic inflammation of the airway mucosa and submucosa. To understand the mechanisms by which BK serves as an inflammatory mediator, the human lung fibroblast cell line WI- 38 was stimulated with BK and the expression of IL-1β gene was examined. BK at nanomolar concentrations induced a marked increase in immunoreactive IL- 1β, detectable within 2 h in both secreted and cell-associated forms. BK- induced IL-1β synthesis was inhibited by a B2-type BK receptor antagonist and by treatment of the cells with pertussis toxin, indicating the involvement of a BK receptor that couples to the G(i)/G(o) class of heterotrimeric G proteins. Whereas cycloheximide and actinomycin D both inhibited BK-induced IL-1β synthesis, results from Northern blot and nuclear run-on assays suggested that BK acted primarily at the transcription level which led to the accumulation of IL-1β message in stimulated cells. Gel mobility shift assays were used with nuclear extracts from stimulated WI-38 cells to examine the transcription mechanism for BK-induced IL-1β expression. A DNA binding activity specific for the decameric κB enhancer was detected and was found to contain the p50 and p65 subunits of the NF- κB/rel protein family. BK-induced NF-κB activation correlated with IL-1β message upregulation with respect to agonist concentration, time course, sensitivity to bacterial toxins, and blockade by the B2 receptor antagonist. After BK stimulation, a significant increase in the activity of chloramphenicol acetyltransferase was observed in WI-38 cells transfected with a reporter plasmid bearing the κB enhancers from the IL-1β gene. Deletion of the κB enhancer sequence significantly reduced BK-induced chloramphenicol acetyltransferase activity. These findings suggests a novel function of BK in the activation of NF-κB and the induction of cytokine gene expression.