Increased percentage of T cells with the expression of CD127 and CD132 in hypertrophic adenoid in children with otitis media with eVusion

Abstract

The hypertrophic adenoid may promote chronic suppurative otitis media in children as it fulWlls its immune function. The number of lymphocytes in the adenoid and their cooperation in the immune response depend of on their proliferation and migration to the eVector sites. Interleukin 7 (IL-7) is essential for the normal development and function lymphocytes. IL-7 plays pivotal role for activation and proliferation of T and B cells. The heterodimeric interleukin- 7 receptor (IL-7R) is composed of the IL-7Rα (127) and the common cytokine receptor γc (CD132). The aim of this study was to evaluate the percentage of lymphocytes T (CD4+ and CD8 +) with IL-7R (CD127 and CD132) expression in hypertrophic adenoid in children suVering with otitis media with eVusion for a duration of 3 months. Adenoid excised due to hypertrophy with or without chronic otitis media with eVusion was used as study material. CD4+ CD127+, CD4 +132+, CD8+CD127+ and CD8 +CD132+ cell subpopulations were identiWed using monoclonal antibodies and Xow cytometry. The percentage of CD4+ and CD8+ T cells with CD127 receptor expression in hypertrophic adenoid of children with otitis media with eVusion was statistically signiWcantly higher than in hypertrophic adenoid group. The percentage of CD4+ T cells with CD132 expression in the study group was statistically signiWcantly higher than in the reference group. The percentage of CD8+ T cells with CD132+ expression was not statistically diVerent in both groups. The increased percentage of T lymphocytes with IL-7R expression (CD127 and CD132) in hypertrophic adenoid seems to inXuence the quantity of lymphocytes and upset the immunological function of tonsils which can inXuence the course of otitis media with eVusion. © Springer-Verlag 2011.