Isolation and Characterization of New Variant Strains of Infectious Bronchitis Virus in Northern Egypt

Abstract

Three strains of infectious bronchitis virus with sever Nephropathological characteristics were isolated from Mansoura, Gharbia and Giza governorates early 2010. Egg propagation revealed the ability of the isolates to make mortalities of the SPF embryos by the 3 rd passage after 72 h post inoculation. The embryos showed stunted growth with sever renal damage and deposition of ureates within the ureters and urinary bladder. PCR amplification of the 976bp of S1 gene gave appositive amplicon with 10 -2 -5 – 10 diluted infected allanotic fluid. phylogenetic analysis revealed that these strains are related to the IS/1494/06 nephropathic IBV strain